Rapeseed Androgenic Haploids: Production and Genetic Transformation

Tissue culture techniques are quite advanced for canola, which has made it an attractive candidate for use in genetic transformation studies. In doubled haploid technology, microspores and microspore-derived embryos are particularly attractive as targets for genetic transformation, due to their haploid nature. Through the application of a wide range of stressing agents, a haploid microspore can be deviated to become a haploid androgenic embryo and, upon chromosome doubling, eventually a doubled haploid plant.