Antioxidant Activity of Euphorbia Helioscopia and Grewia Optiva

In this study total antioxidant capacity (TAC) of Euphorbia helioscopia (EHL) and Grewia optiva (GOL) extracts was investigated. The antioxidant components were initially extracted in methanol and subjected to partitioning in solvents of different polarity. Antioxidant and radical scavenging activity of these extracts were investigated using antioxidant assays such as 2,2´-azinobis(3-ethylbenzothiazoline-6-sulpohonic acid) (ABTS) radical cation scavenging, the ferric reducing antioxidant power (FRAP), 2,2´-diphenyl-1-picrylhydrazil (DPPH) scavenging, total phenolic content (TPC),total flavonoid content (TFC) and total antioxidant activity determination using ferric thiocyanate method, Superoxide anion radical scavenging activity and Metal Chelating activity. Using ABTS•+ decolorization assay and FRAP assay, Euphorbia helioscopia and Grewia optiva extracts showed a wide range of antioxidant activity.