Folding Pathways of Photoactive Proton Pump Bacteriorhodopsin

Folding Pathways of Photoactive Proton Pump Bacteriorhodopsin

A detailed kinetic study involving stop-flow spectroscopy to decipher the folding pathways of Bacteriorhodopsin

LAP Lambert Academic Publishing ( 2010-01-11 )

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Stop-flow fluorescence and photodiode array spectroscopy, with a millisecond time resolution, are used here to investigate refolding kinetics of the photoactive proton pump bacteriorhodopsin in mixed DMPC/CHAPS micelles from a partially denatured state in SDS. The study suggests that both the apoprotein folding and subsequent binding of retinal chromophore likely proceed via distinct multiple parallel pathways to generate the native helical bundle. Taken together, these data should have profound implications on the mechanisms of folding of proteins within biological membranes.

Book Details:

ISBN-13:

978-3-8383-1943-8

ISBN-10:

3838319435

EAN:

9783838319438

Book language:

English

By (author) :

Amjad Farooq

Number of pages:

152

Published on:

2010-01-11

Category:

Biochemistry, biophysics