LAP Lambert Academic Publishing ( 2011-02-07 )
€ 79,00
This research work was performed to examine the feasibility of using Fourier transform infrared (FT-IR) spectroscopy (in the mid-IR range, 4000-400 cm-1) to i) detect and identify bacterial cells. The greatest spectral variation was observed between 1800 cm-1 and 900 cm-1: for amide I band at ~1650 cm-1, amide II band at ~1540 cm-1, CH3 and CH2 of protein components around 1455 cm-1 and 1398 cm-1, P=O of the phosphodiester backbone of nucleic acids around 1242 cm-1 and 1080 cm-1, and polysaccharide compounds around 1050 - 950 cm-1. From the second derivative transformation and loadings plot, FT-IR showed the potential to detect sublethally injured and dead cells and discriminate between different types and degrees of cell injury.
Book Details: |
|
ISBN-13: |
978-3-8383-6060-7 |
ISBN-10: |
3838360605 |
EAN: |
9783838360607 |
Book language: |
English |
By (author) : |
Hamzah Al-Qadiri |
Number of pages: |
204 |
Published on: |
2011-02-07 |
Category: |
Microbiology |