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• In all 15 isolates of Streptomyces were screened for the synthesis of tyrosinase by both qualitative and quantitative methods in synthetic medium. • Activity of tyrosinase, specific activity of tyrosinase, formation of melanin and protein content of tyrosinase were assessed to determine the catalytic power of tyrosinase. • In a batch wise bioprocess, an isolate of Streptomyces DLS-8 has been found to be potential for the production of tyrosinase. • Effect of pH and temperature on the production of tyrosinase were also examined. 300C temperature and pH 8.5 were proved to be optimum for the maximum production of tyrosinase. • Results of the present study revealed that starch and citrulline were the important carbon and nitrogen sources respectively for the maximum production of tyrosinase. • The partial isolation and purification of the tyrosinase was under taken by SDS-PAGE. The tyrosinase was determined to be about 45 KDa, comparing to BSA as standard marker.• Liquid chromatogram mass spectrum reveals the presence of tyrosine, L-Dopa and melanin based on their base peaks for their respective molecular weights indicating significantly the catalytic power of tyrosinase.
Book Details: |
|
ISBN-13: |
978-620-7-47335-9 |
ISBN-10: |
6207473353 |
EAN: |
9786207473359 |
Book language: |
English |
By (author) : |
Raghavendra H |
Number of pages: |
88 |
Published on: |
2024-03-20 |
Category: |
Microbiology |