Determination of the haemagglutinin in influenza vaccines

The book is dedicated to the standardization of methods for the determination of influenza virus haemagglutinin (ectodomain) in the composition of inactivated flu vaccines, the recommendations for the isolation of haemagglutinin as well as methods for calculation by means quantitative microscopy and single radial immunodiffusion method. The author first proposes a new refinement based on geometric construction for calculation of haemagglutinin in single radial immunodiffusion method. The area of a circular ring of precipitation which was formed as a result of precipitation antigen HA with a strain-specific polyclonal antiserum in agarose gel. The amount of antigen present is proportional to the area of the circular ring of precipition.