Evaluation of performance of serological tests

This book describes Evaluation of performance of serological tests for the detection of Foot and Mouth Disease virus antibodies in Kilifi County, Kenya. Objective: To determine the sensitivity of assays Anigen © FMD NSP Ab ELISA –Korea, NSP Priocheck® kits with Liquid Phase Blocking Enzyme Linked Immunosorbent Assay compared with Virus neutralization test ( the gold standard) and Identification of prevalent serotypes of foot and mouth disease virus in Kilifi County coast of Kenya 2014. Design: cross-sectional study design. Setting of the study: Kilifi County. Subjects: 421 cattle serum. Results: The results showed that both Anigen © FMD NSP Ab ELISA (Korea), NSP Priocheck® Netherlands assays had low sensitivity but high specificity. Liquid Phase Blocking ELI SA had the highest sensitivity 100%. LPBE had a Positive Predictive Value (PPV) =100%, Anigen © FMD NSP Ab ELISA–Korea 94.44% and NSP Priocheck® Netherlands had the lowest 44.74% compared with the VNT 100%. The Cohen's kappa coefficient showed a perfect agreement level of 90%, 93%, 100 and 100% respectively. Conclusion: This study showed no significant difference in sensitivity and both structural and non-structural tests.