LAP Lambert Academic Publishing ( 28.07.2015 )
€ 36,90
Petroleum-polluted environmental samples were collected to isolate and characterize biosurfactant-producing bacteria. Culturing of the collected samples in LB-broth and enrichment in chemically defined medium (CDM) with various carbon sources yielded 51 strains, some of which produced biosurfactants/bioemulsifiers when challenged with crude oil as a sole carbon source. The I-15 isolate, a Gram-positive, motile bacillus, emulsified crude oil in CDM without reduction in surface tension. Infrared spectroscopy and thin layer chromatography suggested a lipopeptide structure for the crude biosurfactant.Partial sequences of 10 16S rDNA gene clones from the I-15 strain were highly similar to those of various members of the family Bacillaceae. The I-15 strain is a promising biosurfactant producer and is probably an active indigenous crude oil degrader. Biosurfactant production is accompanied by morphological and physiological alterations. The I-15 strain possesses intragenomic heterogeneity in the rrn (RNA) operons.
Kitap detayları: |
|
ISBN-13: |
978-3-659-29112-8 |
ISBN-10: |
3659291129 |
EAN: |
9783659291128 |
Kitabın dili: |
English |
Yazar: |
Israa S. Al-Rowaihi |
Sayfa sayısı: |
144 |
Yayın tarihi: |
28.07.2015 |
Kategori: |
Mikrobiyoloji |