Cloning and Expression of a Thermostable Lipase

The expression vector and host are important factors for achieving maximal expression of cloned genes however; molecular cloning of a foreign gene does not ensure that the gene will be expressed successfully. The most difficult problems in bacterial expression are proteolytic degradation and production of proteins that accumulate in misfolded forms, most often as inclusion bodies.Over the past decade, there is an interest in thermophilic endospore forming bacteria of the genus Bacillus, because of their possible contamination of heated food products and of their biotechnological importance as sources of thermostable enzymes and other products of industrial interest.